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PM 2.5 and PM 2.5-10 induced cell migration in <t>HPASMCs,</t> MPASMCs, and MVSMCs. HPASMCs were treated with d 2 H 2 O or 25 μg/mL PM 2.5 or PM 2.5-10 for 48 h. Migration abilities of HPASMCs, MPASMCs, and MVSMCs were measured, quantified and shown as ( A , B , D , E , G , H ). The relative levels of SMA mRNA for HPASMCs, MPASMCs, and MVSMCs were determined using a real-time PCR assay, and shown as ( C , F , I ). ( J ) MVSMCs were stained with Alexa Fluor 546-Phalloidin.(red) to identify lamellipodia (white arrows). The results are presented as the mean ± SD for three or four independent experiments. * p < 0.05, compared with d 2 H 2 O-treated cells. Con: d 2 H 2 O treatment as control.
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PM 2.5 and PM 2.5-10 induced cell migration in HPASMCs, MPASMCs, and MVSMCs. HPASMCs were treated with d 2 H 2 O or 25 μg/mL PM 2.5 or PM 2.5-10 for 48 h. Migration abilities of HPASMCs, MPASMCs, and MVSMCs were measured, quantified and shown as ( A , B , D , E , G , H ). The relative levels of SMA mRNA for HPASMCs, MPASMCs, and MVSMCs were determined using a real-time PCR assay, and shown as ( C , F , I ). ( J ) MVSMCs were stained with Alexa Fluor 546-Phalloidin.(red) to identify lamellipodia (white arrows). The results are presented as the mean ± SD for three or four independent experiments. * p < 0.05, compared with d 2 H 2 O-treated cells. Con: d 2 H 2 O treatment as control.

Journal: Antioxidants

Article Title: Ambient Particulate Matter Induces Vascular Smooth Muscle Cell Phenotypic Changes via NOX1/ROS/NF-κB Dependent and Independent Pathways: Protective Effects of Polyphenols

doi: 10.3390/antiox10050782

Figure Lengend Snippet: PM 2.5 and PM 2.5-10 induced cell migration in HPASMCs, MPASMCs, and MVSMCs. HPASMCs were treated with d 2 H 2 O or 25 μg/mL PM 2.5 or PM 2.5-10 for 48 h. Migration abilities of HPASMCs, MPASMCs, and MVSMCs were measured, quantified and shown as ( A , B , D , E , G , H ). The relative levels of SMA mRNA for HPASMCs, MPASMCs, and MVSMCs were determined using a real-time PCR assay, and shown as ( C , F , I ). ( J ) MVSMCs were stained with Alexa Fluor 546-Phalloidin.(red) to identify lamellipodia (white arrows). The results are presented as the mean ± SD for three or four independent experiments. * p < 0.05, compared with d 2 H 2 O-treated cells. Con: d 2 H 2 O treatment as control.

Article Snippet: Human pulmonary artery SMCs (HPASMCs) were purchased from Lonza (Walkersville, MD, USA) (Cat# CC2581) and cultured in SMC medium (Lonza, Walkersville, MD, USA; cat# CC3182).

Techniques: Migration, Real-time Polymerase Chain Reaction, Staining, Control